Two-tailed unpaired t-tests were used to assess differences in the lung
CYP2A protein levels between treatment groups in AGM study 1. Two-way
ANOVA tests were used to assess differences in the lung CYP2A protein
levels between treatment groups in AGM study 2. Tukey’s multiple
comparison post hoc tests were subsequently used to assess differences
in lung CYP2A protein levels between treatment groups and treatment
interactions in AGM study 2. The D’Agostino-Pearson omnibus normality
test was used to determine the normality of signal distribution,
followed by correlation analyses to assess the relationships between
lower and upper lung CYP2A protein levels within individual AGMs. Data
from both AGM studies were found to be normally distributed; thus,
Pearson’s correlations were used to assess the relationship between the
lower and upper lung.