Materials
Human liver microsomes were prepared in a previous study (Al Koudsi et
al., 2010). Microsomes were prepared as a pool of 17 livers from the
Biocentre in Basel, Switzerland (23). AGM liver microsomes were prepared
in a previous study (Ferguson et al., 2011). Microsomes were prepared as
a pool of 10 livers from the Control group AGM from Study 2.
Human CYP2A13 (Cat #: EZ033) and CYP2D6 (Cat #: EZ013) bactosomes were
purchased from SEKISUI XenoTech (KS, USA). Human 2A6 enzyme microsomes
(Cat #: 456204) were purchased from CORNING Inc. (NY, USA). Human
CYP2B6 (Cat #: 456255), CYP3A4 (Cat #: 455307) and CYP2E1 (Cat #:
456255) enzyme microsomes were purchased from BD Bioscience Company (MA,
USA). All other reagents were obtained from standard suppliers.
Information about the antibodies used can be found in Supplementary
Table 3.