CYP2A protein levels were detected in AGM lung by immunoblotting
An immunoblotting assay was established using a polyclonal anti-CYP2A6 antibody to measure the levels of CYP2A protein in AGM lung. A CYP2A immunoreactive signal was detected in human liver, AGM liver, AGM lung, and cDNA-expressed CYP2A13 and CYP2A6 (Figure 4A), while there was no cross-reactivity with other cDNA-expressed human CYPs (CYP3A4, CYP2E1, CYP2D6, and CYP2B6) (Figure 4A). The assays were linear for detection of CYP2A, β–actin and Coomassie blue signals over a wide range of AGM lung protein (Figure 4B–D).