2.3.1 Capsule oil content and fatty acids composition
For the oil content of the capsule, dry capsules were ground and 2 grams of sample were extracted in a Soxhlet apparatus containing 80 mL of petroleum ether as a solvent. The solvent was then evaporated and the oil content of the sample was determined gravimetrically (AOAC, 2005). 0.1 g oil sample was dissolved in 5 ml hexane. 0.5 ml 0.2N KOH (prepared in methanol) was added. After shaking well, it was kept in the dark for a while. Centrifugation was applied. 1 ml was taken from the upper phase and placed in the vials. Finally, the device was injected with a syringe. The fatty acid compositions of the oil were detected with GC/MS-QP 2010 (Schimadzu) in the Science and Technology Application and Research Center of Yozgat Bozok University (Turkey). The chromatographic method is given in Table 1.