Screening of differentially expressed metabolic genes related to survival
We obtained a total of 398 COAD samples and 39 normal colon samples from TCGA for gene expression analysis and prognostic analysis, and downloaded a dataset containing 1048 COAD samples (GSE40976) from the GEO database. Downloaded the KEGG gene set c2.cp.kegg.v7.0.symbols.gmt containing metabolic genes from the GSEA website, and extracted a total of 921 Metabolism-related genes. Then, 645 cross-expressed genes were screened in TCGA-COAD and GSE40976. P Value <0.05 and |log2-fold change|> 0.5 were defined as DEG. A heat map analysis was performed to show cluster analysis of gene characteristics (Figure 1A), and a volcano map was constructed to reveal 225 significantly DEG (Figure 1B). A total of 377 cases were extracted from TCGA-COAD, and 556 cases were extracted from GSE40976 for prognostic analysis. Delete missing data and cases with survival time less than 30 days from the cases. Univariate Cox regression analysis revealed 22 PRMG in TCGA-COAD (Fig. 1C, p value <0.05).