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A Luminex based serological assay for detecting IgM and IgG antibody response in SARS-CoV2 patients
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  • Shailendra Mani,
  • Tripti Shrivastava ,
  • Sankar Bhattacharyya ,
  • Nagender Rameshwaram ,
  • Sweety Samal,
  • Sandeep Goswami ,
  • Ashok Kumar,
  • Ramandeep Singh,
  • Niraj Kumar,
  • Nitya Wadhwa ,
  • Uma Chandra Natchu ,
  • Ramachandran Thiruvengadam ,
  • Shailaja Sopory ,
  • Pallavi Kshetrapal ,
  • Bapu Desiraju ,
  • Vandita Bhartia
Shailendra Mani
Translational Health Science and Technology Institute
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Tripti Shrivastava
Translational Health Science and Technology Institute
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Sankar Bhattacharyya
Translational Health Science and Technology Institute
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Nagender Rameshwaram
Translational Health Science and Technology Institute
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Sweety Samal
Translational Health Science and Technology Institute
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Sandeep Goswami
Translational Health Science and Technology Institute
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Ashok Kumar
Translational Health Science and Technology Institute
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Ramandeep Singh
Translational Health Science and Technology Institute
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Niraj Kumar
Translational Health Science and Technology Institute
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Nitya Wadhwa
Translational Health Science and Technology Institute
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Uma Chandra Natchu
Translational Health Science and Technology Institute
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Ramachandran Thiruvengadam
Translational Health Science and Technology Institute
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Shailaja Sopory
Translational Health Science and Technology Institute
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Pallavi Kshetrapal
Translational Health Science and Technology Institute
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Bapu Desiraju
Translational Health Science and Technology Institute
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Vandita Bhartia
Translational Health Science and Technology Institute
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Abstract

Nucleic acid real-time PCR test has become the main diagnostic tool for SARS‐CoV‐2 infection, and there are also drawbacks to these real-time PCR test kits. Additionally, it has reported high false-negative rates. A reliable and rapid test method is urgently required to briskly detect antibody response in a large number of infected patients and ensure prompt care for patients. Therefore, we report here a Luminex bead based multiplexed immunoassay to serologically diagnose the SARS-CoV2 infection and detect IgM and IgG response in COVID-19 patients. The technology can provide sensitive and specific detection using a low amount of serum samples in high-throughput screening platforms.