Detection of IgG1 and IgG2a in serum by ELISA
Serum samples were collected 14 days after C. rodentiuminfection. The changes in IgG1 and IgG2a levels in serum were assessed
using an ELISA kit (Bioss, CHN) following the manufacturer’s
instructions for cytokine detection. Cytokine levels were expressed per
milligram of total protein. All data were obtained from three separate
experiments.