Experimental grouping
The experimental mice were randomly divided into four groups, with more
than nine mice in each group: the control group (PBS), the T.
spiralis -infected group (TS), the C. rodentium -induced colitis
model group (CR), and the T. spiralis -pre-infected C.
rodentium -induced colitis group (TS+CR). At the beginning of the
experiment, each mouse in the TS and TS+CR groups was orally infected
with 400 individual T. spiralis muscle larvae. After 21 days of
infection, the control and TS groups were administered PBS, and the CR
and TS+CR groups were administered 2×109 CFU/mLC. rodentium . Subsequently, the faeces of mice in the CR and
TS+CR groups were collected every day from days 1 to 14 post C.
rodentium infection, the colony count of faeces was determined, and the
number of C. rodentium per gram of faeces (CFU/g) was calculated.
The mice were sacrificed under euthanasia on days 7 and 14, and the
weights of the mice in the four groups was determined daily.