Disease activity index evaluation
The mice were observed daily in terms of the changes in their mental
status, activity, hair gloss, appetite, and defecation (presence of
blood, stool pattern, defecation frequency). Each group of mice was
scored for the disease activity
index (DAI) according to international standards: DAI = (weight loss
score + stool score + blood stool score)/3. To detect faecal occult
blood, we used the benzidine method.