10. Figure legends
Figure 1: Hepatic
DNA methylation landscape in K. marmoratus . A: Frequency
distribution of MSPI fragment size. Mean fragment size was 95 bp and
median 88 bp; B: Overall distribution of analysed fragments in genomic
elements; C: Frequency distribution of DNA methylation % for MSPI
fragments. Distribution of MSPI fragments follows a bimodal
distribution, which is expected given the binary character of DNA
methylation in cells; D: Boxplot of DNA methylation (%) of fragments
according to their location in genomic elements (including medians and
quantile 25-75%).
Figure 2: Distribution of lowly methylated (<20% methylation)
(A) and highly methylated (> 80% methylation) (B)
fragments among the genomic regions (gene body, promoter, upstream,
intergenic) of the liver reduced representative genome of the mangrove
rivulus.
Figure 3: A: Volcano plot representations of significance and
differential methylation in analysed fragments. Dotted lines represent
thresholds of significance: -10LogP20, corresponding to P0.01 and mean
methylation difference 10% A: 4 ng/L vs control; B:120 ng/L vs control;
C: 4 ng/L vs 120 ng/L. B: Overall distribution of DMFs in genomic
elements; C: Venn Diagram showing specific and common DMFs between group
comparisons; D: Methylation levels (%) of common fragments between 4 vs
Ctl and 120 vs Ctl comparisons (Da,Db), between 4 vs Ctl and 4 vs 120
comparisons (c,d,e) and between 120 vs Ctl and 4 vs 120 ng/L comparisons
(f). For clearer representation of methylation differences, y axes start
at 40 %.
Figure 4: Network of genes corresponding to significant DMFs (located in
promoter region or in gene body) at 4 ng/L compared to control. Blue:
hypermethylation at 4 ng/L compared to control, Yellow: hypomethylation
at 4 ng/L compared to control.
11. Figures