METHODOLOGY
The present study was undertaken in Shimogga district during the year 2019-2020. The Terrestrial Orchids of eight different Habenariaspecies , were collected at different regions from Shivamogga district at different vegetation types includes evergreen , deciduous and open forest type.( Table 1.) Habenaria species, were identified with the help of available manuals and floras ( Gamble ,1935; Rao , 1998). To evaluate the leaf anatomy, samples were fixed in FAA 50% for 48 hours and then transferred to Ethanol 50% (Johansen ,1940). Histological slides were prepared by freehand section. Each one was sectioned in the midline, free hand sections were obtained using a razor blade, Transverse sections were subjected to staining with 1% safranin (Kraus and Arduin, 1997). Leaf sections were analyzed in light microscope. In leaf section number of layers in epidermis, hypodermis, mesophyll and vascular bundles, were observed and noted. The epidermis of leaves were peeled from the fresh leaves to observe structure of the stomata. The most important aspects were recorded with digital camera attached to Olympus microscope.