Determination of optimum pH for maximum zinc absorbed group and examination of growth rate as well as Zrt1 and Fet4 expression level in this pH
After determination of absorbed zinc by yeast biomass in all zinc treated groups, a yeast biomass with maximum growth rate, absorbed zinc, and expression level of Zrt1 and Fet4 in yeast cells, were selected. This group was considered to study at pH 3, 4, 5 and 6, under conditions that mentioned in the previous section, to find the optimum pH for maximum zinc absorption. In the following, estimation of growth rate and total accumulation of zinc in yeast cells as well as determination of Zrt1 and Fet4 expression level in zinc absorbed yeasts were performed as mentioned earlier (spectrophotometry, CDW estimation, and qRT-PCR).