Table 2: Number of PCR replicates required to reach the point at which taxon accumulation curve (shown in Figure 5) increases by less than one taxon with the addition of another PCR replicate. We observe variation by site and marker, and a common trend of decreasing replicates required with an increase in read cutoff. ‘>X’ denotes that greater than the maximum number of replicates X retained after rarefaction is needed to suffice this point.