Figure 6. Histograms describing the frequency of individual taxa detected across PCR replicates, each sampled to a read depth of 5000 reads and using a minimum read cutoff of five, out of the total 24 replicates. The right-most bar is a count of taxa present in all replicates while the left-most bar is a count of taxa present in only one replicate. Increasing the minimum read cutoff to ten reduced the number of singleton taxa, while decreasing the minimum read cutoff to two increased the number of singleton taxa (data not shown).