2.3 Preparation and collection of free and glycosyl-linked endogenous scent compounds
The free and de-glycosylated endogenous volatiles were collected from the flowers grown under different temperature conditions upon using the method as described earlier by Barman and Mitra (2019). After extracting the emitted volatiles, flowers were plucked from the plants and frozen immediately in liquid nitrogen to get the free endogenous volatiles. The volatiles were collected by powdering the harvested flowers and extracting in DCM solvent. The glycosyl-linked endogenous volatiles were collected from petal residues of mature buds grown under different temperature. Hydrolysis of bound glycosyl moiety from volatile compounds was carried out with the help of 200 µl of viscozyme®L (Sigma-Aldrich). The de-glycosylated volatiles were extracted in DCM. The water molecules from the extract were removed by using anhydrous sodium sulphate.