2.13 Statistical analysis
The mean and standard deviation values with a minimum of three replicate
data set were calculated using Microsoft Excel 2019. To find the
significance of difference amongst the samples, statistical analysis
such as Tukey’s HSD test was performed using Past3 software (p
< 0.05). Principal component analysis (PCA) from metabolite
data was achieved via MetaboAnalyst 4.0, an open source software (Xia et
al., 2015).