2.3 Preparation and collection of free and glycosyl-linked
endogenous scent compounds
The free and de-glycosylated endogenous volatiles were collected from
the flowers grown under different temperature conditions upon using the
method as described earlier by Barman and Mitra (2019). After extracting
the emitted volatiles, flowers were plucked from the plants and frozen
immediately in liquid nitrogen to get the free endogenous volatiles. The
volatiles were collected by powdering the harvested flowers and
extracting in DCM solvent. The glycosyl-linked endogenous volatiles were
collected from petal residues of mature buds grown under different
temperature. Hydrolysis of bound glycosyl moiety from volatile compounds
was carried out with the help of 200 µl of viscozyme®L
(Sigma-Aldrich). The de-glycosylated volatiles were extracted in DCM.
The water molecules from the extract were removed by using anhydrous
sodium sulphate.