Purpose: The purpose of this study is to analyze the possible mediator effect of mindfulness in the relationship between perceived stress and quality of life in pediatric asthma. Methods: The sample of this study consisted of 100 asthmatic children aged between 9-12 years who applied to İstanbul University İstanbul Medical Faculty Hospital’s “Child Allergy Policlinic”. Sociodemographic information forms, Perceived Stress Scale in Children (8-11 years), Child and Adolescent Mindfulness Measure (CAMM) and Pediatric Asthma Quality of Life Questionnaire (PAQLQ) were used as data collection tools. Results: Linear hierarchical regression analysis was used during the process of analyzing data. It has been identified that mindfulness has a partial mediator role, in the relationship between perceived stress and quality of life (p=-,474), in the relationship between perceived stress and symptoms which is the subscale of quality of life (p=-,399) and in the relationship between perceived stress and emotional function (p=-,514) which is the quality of life’s subscale. The mediating role of mindfulness in the relationship between perceived stress and activity limitations could not been inspected due to the lack of a significant correlation between activity limitations which is subscale of quality of life and mindfulness (p=,136). Conclusions: When all these findings are evaluated, it might be beneficial for experts dealing with pediatric asthma to add psychotherapy interventions involving mindfulness practices to the treatment of the disease.