Objective: To investigate the clinical significance of dynamically monitoring serum estradiol (E2) and β-human chorionic gonadotropin (β-HCG) in early pregnancy assessment and their values in predicting pregnancy outcomes.. Methods: In this retrospective study, two hundred early pregnancy women were divided into two groups according to their early pregnancy outcomes: the ongoing pregnancy group and inevitable abortion group. Serum E2 and β-HCG levels and their growth rates were compared weekly. Results: E2 and β-HCG of the ongoing pregnancy group were significantly higher than that of the inevitable abortion group from the 5th to 10th week of pregnancy. Taking 489.5 pg/ml in the 5th and 6th week, 590.5 pg/ml in the 7th week and 614.5 pg/ml in the 8th week as cut-off levels of E2, the sensitivity and specificity for E2 to predict bad pregnancy outcome were 91.7% and 41.5%, 82.9% and 71.1%, 84.8% and 84.7%, 75.0% and 95.7%, respectively (P< 0.05). Both E2 and β-HCG increased much more rapidly in the ongoing pregnancy group. 80% of the normal pregnancy women showed continuously increasing E2 level. Meanwhile, the inevitable abortion group presented E2 variation types as slow increase or fluctuation, continuous decline, and sudden drop, which account for 54.0%, 34.0%, and 12.0%, respectively. Conclusion: Low values and low growth rates of E2 and β-HCG probably indicate bad pregnancy outcomes.