miR-195-5p regulated CORM-3-induced osteoblastic differentiation of rat BMSCs by targeting Wnt3a
To verify whether the effect of miR-195-5p during CORM-3-induced osteoblastic differentiation depended on Wnt3a, rat BMSCs were co-transfected with miR-195-5p mimics and pcDNA3.1-Wnt3a or vector NC. With miR-195-5p up-regulated, the mRNA and protein expressions of Runx2 and OPN were increased after Wnt3a overexpression on the 3rd and the 7th day of osteogenic induction (P < 0.05) (Fig. 6A-B). Matrix mineralization was significantly decreased with miR-195-5p mimics transfection on the 14th day of osteogenic induction. However, the decreased matrix mineralization was rescued by the overexpression of Wnt3a (Fig. 6C), which indicated that miR-195-5p negatively regulated CORM-3-induced osteogenic differentiation of rat BMSCs through directly targeting Wnt3a.