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Latent role of RPL26 in regulating respiratory edema of chronic rhinosinusitis with nasal polyps
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  • Chuanliang Zhao ,
  • Jiaxiong Zhang,
  • Wentao Zou,
  • Zimu Zhang,
  • Minlin Jiang,
  • Xiaojing Cai,
  • Shaoqing YU
Chuanliang Zhao
Tongji University Tenth People's Hospital
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Jiaxiong Zhang
Tongji University Tenth People's Hospital
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Wentao Zou
Tongji University Tenth People's Hospital
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Zimu Zhang
Tongji University
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Minlin Jiang
Tongji University
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Xiaojing Cai
Tongji University Tenth People's Hospital
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Shaoqing YU
Tongji Hospital, Tongji University, Shanghai, China
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Abstract

Backgroud: In order to study how to reduce the edema of CRSwNP, we tried to co-cultured CRSwNP with glucocorticoid, predicted and verified possible mechanisms through gene sequencing and CRISPR-Case9 gene editing technology Method:We co-cultured CRSwNP with glucocorticoid , and selected normal CRSwNP as control group. Then analyzed the differentially expressed genes(DEGs), and the RPL26 gene was found through bioinformatics analysis. CRISPR-Case9 was used to establish the RPL26 gene silencing and overexpression cells system; Immunofluorescence staining and flow cytometry analysis were used to observe changes in cell morphology and apoptosis. Results: Pearson Correlation to analyze the correlation of the probe signal values of all specimens and found that the correlation between the two sets of experimental data was high, R value was close to 1. A total of 12336 genes were detected, and 5026 genes were differentially expressed. Then screened sequence length of ≤1000, 916 of DEGs were obtained, including 340 up-regulated genes and 576 down-regulated genes. Via bioinformatics analysis, the Ribosome pathway is most relevant, and the RPL26 plays an important role. The results of immunofluorescence staining and apoptosis experiments showed that high expression of RPL26 can effectively reduce the degree of edema of CRSwNP cells, and has little effect on apoptosis. Conclusion: Highly expressed RPL26 can effectively reduce the edema of CRSwNP cells, proving that RPL26 plays an important role in this mechanism, and this role of RPL26 may be highly correlated with the ribosome signaling pathway.