Cell apoptosis was analyzed by flow cytometry
Part of the samples were digested by trypsin without EDTA in each group and then collected by centrifugation. After repeated washing with PBS, cell resuspension solution was added for resuscitation, then apoptotic solution (5ul FITC staining solution and 10ul PI staining solution) was added, and the cells were left at 37℃ darkroom for 10min. After adding cell resuspension solution again, the samples were measured by computer to count the apoptosis rate.