Construction of RPL26 silencing and overexpression cell model
Both RPL26 silencing and RPL26 overexpression lentivirus transfection
plasmids were constructed with the help of the basic medical laboratory
of Tongji University Medical School. CRSwNP cells at logarithmic growth
stage were randomly divided into three groups, and then performed RPL26
gene silencing and overexpression transfection: Group A was transfected
with blank plasmid(Control); Group B was transfected with RPL26
silencing plasmid(anti-RPL26); Group B was transfected with RPL26
overexpression plasmid(pre-RPL26). The cells were amplified for 3 days.