Transcriptome sequencing
The Agilent used in this experiment was Mouse lncRNA Microrray V3
(4*180K, Design ID: 084388) . Data analysis were conducted by OE
Biotechnology Co., Ltd., (Shanghai, China). the NanoDrop ND-2000 (Thermo
Scientific) was used to quantifed Total RNAļ¼and the RNA integrity was
assessed by Agilent Bioanalyzer 2100 (Agilent Technologies). The sample
labeling, microarray hybridization and washing were performed based on
the standard protocols. Total RNA were transcribed to double strand
cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP. The
labeled cRNAs were hybridized onto the microarray. After washing, the
arrays were scanned by the Agilent Scanner G2505C (Agilent
Technologies). Feature Extraction software (version10.7.1.1, Agilent
Technologies) was used to analyze array images to get raw data.
Genespring (version 14.8, Agilent Technologies) were employed to finish
the basic analysis with the raw data.