Conclusion:
We demonstrated that rapid and accurate detection of Bd in frog skin swabs can be accomplished using field extraction and analysis techniques with a mobile real-time PCR device. The methods applied worked for presence/ absence detection data of high-quality (swab) samples. The field-based DNA extraction and qPCR analysis is a promising management tool to aid in the recovery of declining amphibian species. However, there are tradeoff between using the rapid in-the-field methods as traditional lab methods are more sensitive to low quantities of DNA (Sepulveda et al., 2018). Currently, our methods could not be used for accurate DNA quantification and should be used cautiously when detecting low quality DNA samples (i.e. eDNA) especially when the target specimen is present a low densities. Additionally, scope of operation should be considered as it may be more cost and time efficient to run high volumes of samples using lab-based approaches.