3.4 PsbA and PsbS photosynthetic gene expression
Expression analysis of PsbA and PsbS genes showed that treatment of
wheat and cucumber plants by amaranth leachate caused a change in the
expression of these genes, which also differed between studied species
(Figure 1C). Leachate treatment significantly decreased PsbA gene
expression in cucumber, but this gene expression was significantly
stimulated in wheat plants (p ≥ 0.01). PsbS gene expression in
both cucumber and wheat plants reduced by application of amaranth
leachate, but it was only statistically significant in the cucumber
plant (p ≥ 0.01). This difference was also seen in the gel images
of RT-PCR products of the studied genes (Figure 1B).