Material
Diosmetin was obtained from Shanghai yuanye Bio-Technology Co., Ltd.
(Shanghai, China). LPS was
purchased from Santa Cruz
Biotechnology. (Santa Cruz, USA). The BCA Protein Assay Kit, DCFH-DA,
GSH-Px, GSH, MDA and SOD kits were obtained from Beyotime Institute of
Biotechnology (Shanghai, China). The First-Strand cDNA Synthesis
SuperMix kit and Green qPCR SuperMix kit were obtained from TransGen
Biotech (Beijing, China). The MitoSOX Red Mitochondrial Superoxide
Indicator was purchased from Yesen (Beijing, China). Anti-Occludin,
anti-Claudin-1 and anti-ZO-1 were
obtained from Proteintech Group, Inc. (Chicago, IL, USA). Anti-GAPDH
were obtained from Cell Signaling Technology (Boston, United States).