Material
Diosmetin was obtained from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). LPS was purchased from Santa Cruz Biotechnology. (Santa Cruz, USA). The BCA Protein Assay Kit, DCFH-DA, GSH-Px, GSH, MDA and SOD kits were obtained from Beyotime Institute of Biotechnology (Shanghai, China). The First-Strand cDNA Synthesis SuperMix kit and Green qPCR SuperMix kit were obtained from TransGen Biotech (Beijing, China). The MitoSOX Red Mitochondrial Superoxide Indicator was purchased from Yesen (Beijing, China). Anti-Occludin, anti-Claudin-1 and anti-ZO-1 were obtained from Proteintech Group, Inc. (Chicago, IL, USA). Anti-GAPDH were obtained from Cell Signaling Technology (Boston, United States).