KEY RESULTS
The results showed that diosmetin can markedly decrease the disease
activity index and microscopic colon tissue damage, increase the
expression of tight junction protein (Occludin, Claudin-1 and
Zo-1) and reduce the secretion of
proinflammatory cytokines. And diosmetin also significantly inhibited
colon oxidative damage through adjusting the levels of intracellular
ROS, mitochondrial ROS, GSH-Px, SOD, MDA and GSH in vitro and in vivo.
Furthermore, it was found that
diosmetin can modulate the
abundance of Bacteroidetes, Actinobacteria, Cyanobacteria andFirmicutes, which were reported to be the crucial bacteria
related to inflammatory bowel disease (IBD). Also, diosmetin
significantly increased the expression of Nrf2 and HO-1 and reduced the
ratio of acetylated NF-κB and NF-κB by activating the circ-Sirt1/Sirt1
axis, thereby inhibiting oxidative stress and inflammation.