Histological assessment
The isolated colon tissues were fixed in 10% formalin for one week,
embedded in paraffin, and sectioned into 5 µm slices, and then the
sections were stained with hematoxylin-eosin (H&E) solution. Finally,
images of the stained sections were
photographed using a light
microscope (Nikon Eclipse TE2000-U, NIKON, Japan) at 200 ×
magnification.