DSS-induced IBD
The mice were divided into five
groups (n = 10): Control group
(0.5% CMC-Na), DSS group (the
concentration of 3%), DSS with salazosulfapyridine (SASP, 200 mg/kg/d)
group and DSS with
diosmetin (25 and 50 mg/kg/d)
treatment groups. In the DSS, DSS with SASP
and DSS with diosmetin groups, the
mice were given 3% DSS (molecular weight of 36–50 kDa, MP Biomedical
Solon, OH, USA) in drinking water on days 0–7 and the daily
drinking volume of each mouse is
calculated as 6mL, and the DSS solution is supplemented to the daily
drinking volume the next day., while in the Control group, the mice were
given drinking water without DSS.
In the DSS with SASP and DSS with
diosmetin groups, the mice were given SASP (200mg/kg/d) and diosmetin
(25 and 50 mg/kg/d) on the day 8-15, and the mice in the NC and DSS
groups were given 0.5% CMC-Na. The second model was also divided into
five groups (n = 10): Control group (0.5% CMC-Na), DSS group (the
concentration of 3%), DSS with salazosulfapyridine (SASP, 200mg/kg/d)
group and DSS with diosmetin (25 and 50 mg/kg/d) treatment groups. In
the DSS, DSS with SASP and DSS with diosmetin groups, the mice were
given 5% DSS in drinking water on the day 0–7, while in the Control
group, the mice were given drinking water without DSS. In the DSS with
SASP and DSS with diosmetin groups, the mice were given SASP (50mg/kg/d)
and diosmetin (25 and 50 mg/kg/d)
on the day 1–7, and the mice in the NC and DSS groups were given 0.5%
CMC-Na. During the experiments, body weight and disease activity index
(DAI) (13) were measured daily. After mice were sacrificed, the colon
tissue and feces samples were collected and colon length was measured.