Quantitative real-time PCR assay
Total RNA samples were obtained from colon tissues using TRIzol
(Invitrogen) reagent following the manufacturer’s protocol. cDNA was
synthesized by reverse transcription of each RNA sample by First-Strand
cDNA Synthesis SuperMix (TransGen
Biotech, Beijing). The levels of proinflammatory factor were quantified
using Green qPCR SuperMix (TransGen
Biotech, Beijing). The GAPDH RNA was used for normalization. The primers
used in the present study are indicated in Supporting Information Table
S1