Cell viability assay
The Caco2 and IEC-6 cells were
seeded in 96-well plates at a density of 5 × 104cells·mL-1 for 24 h and pretreated with
various concentrations of
diosmetin (0- 400
μM·mL-1) for 6, 12
and 24 h and various concentrations of Lipopolysaccharide (LPS, 0-32
μg·mL-1). Cell viability was measured by the MTT method as described
above.