Microbial community analysis
Mice feces were collected and stored at −80 °C. The mice feces were divided into three groups (n = 5): Control group,DSS group and DSS with diosmetin (50 mg/kg) group, and the 16S rDNA gene sequencing of the feces samples were sent to Center for Genetic & Genomic Analysis, Microread, (Beijing, China) under dry ice conditions. The DNA of total bacteria in mice feces was extracted with QIAamp® Fast DNA Stool Mini Kit. Primers corresponding to V3-V4 regions of bacterial 16S rRNA were selected for amplification. Sequencing results and statistical results of sequence data of all samples were based on sequenced reads and operational taxonomic units (OTUs).