Microbial community analysis
Mice feces were collected and stored at −80 °C. The mice feces were
divided into three groups (n = 5): Control group,DSS group and DSS with
diosmetin (50 mg/kg) group, and the 16S rDNA gene sequencing of the
feces samples were sent to Center for Genetic & Genomic Analysis,
Microread, (Beijing, China) under dry ice conditions. The DNA of total
bacteria in mice feces was extracted with QIAamp® Fast DNA Stool Mini
Kit. Primers corresponding to V3-V4 regions of bacterial 16S rRNA were
selected for amplification. Sequencing results and statistical results
of sequence data of all samples were based on sequenced reads and
operational taxonomic units (OTUs).