Data and Sample Collection
After consent or assent with parental consent was obtained from survivors and controls, participants filled out a brief history regarding health history, diet, and exercise. Body weight, height, and blood pressure were obtained by clinic staff. Clinical lab testing was performed as indicated for survivors. Serum, plasma, and whole blood were drawn for later analysis for all subjects at study visit. Stool was collected at the patient’s home or on-site at the Cleveland Clinic using an Abbexa (Houston, TX) Feces Catcher stool collection kit, then shipped on ice to the Genomics Medicine Biorepository within 30 days. The samples were then aliquoted and centrifuged at 13,200rpm for 15s. The supernatant was removed, and the fecal pellet was stored at -80°C to await further processing.