Biomarker Laboratory Analysis
Lipid and HbA1c measurements were obtained in a Clinical Laboratory
Improvement Amendments certified lab, if clinically indicated. If these
lab tests were not clinically indicated at the time of the visit, stored
samples were then later run on a similar machine at the Cleveland Clinic
Laboratory Diagnostic Core (Cobas ce6000 e601 module and c501 module).
Additional biomarker assays were also performed at the Cleveland Clinic
Laboratory Diagnostic Core. C-Reactive protein (CRP) was measured using
the Cobas ce6000 e601 module. The automated Tecan EVO Liquid/Plate
System was utilized for for Enzyme Linked Immunosorbant assays (ELISA).
ELISA were used to measure Adiponectin, Leptin, Tumor Necrosis Factor
alpha, Interleukin-6, and Interleukin-10 (R&D Systems, Minneapolis,
MN).