Figure 3. Mutation of PRDM16 decrease brown
adipogenesis. (A ) The mRNA level of PRDM16 . (B) The
mRNA level of PPARγ at fully differentiation. (C )
Protein expression of PRDM16 and PPARγ. (D ) mRNA levels of
brown fat-selective genes analyzed. (E ) Protein expression of
UCP1. Data are means ± SE. (A, B, E) n=5–6/group; (C, F )
n=3/group. One-way ANOVA with Tukey post-hoc test was used to compare
groups. *P < 0.05, **P < 0.01,
***P < 0.001.