Plant Material and Growth Conditions
Seeds of two independent transgenic barley (Hordeum vulgare L.
cv. Golden Promise) lines (W1-1 and W1-7) with RNAi knockdown of theWHIRLY1 gene and wild type controls were produced as described
previously (Krupinska et al., 2002). Barley seeds (1 per pot) were sown
in pots in compost (SHL professional potting compost) in controlled
environment chambers with a 16h light/8h dark photoperiod, with an
irradiance of 250 μmol m-2s-1,
20ºC/16ºC day/night temperature regime and 60% relative humidity. The
primary leaves were harvested after 7 days.