Legend to the figures
Figure 1: Delivery of cashew allergens by epicutaneous patches leads to enhanced allergen capture by skin DCs in sensitized mice. Mice orally sensitized to cashew or naïve mice received a patch loaded with cashew-F647 (in green) or a patch containing excipient as a negative control (in white), for 6 hours. Skin samples were collected, and cells were isolated and labelled for FACS analysis. The percentage of F647-positive cells was measured among each skin DC subsets, (n = 8 per group). Data are median and interquartile ranges. P values were determined according to the Mann-Whitney test (**, p<0.01; ***, p<0.001).
Figure 2: Cashew-positive skin DC isolated in cashew-sensitized mice show an increased expression of PD-L2 and a concomitant decrease of CD86 expression. Mice were treated as described in Figure 1 and skin cells were analyzed by FACS. The expression of PD-L2 and CD86 was evaluated in F647-positive cells by measuring the median of fluorescence intensity of each marker. (n = 8 per group). Data are median and interquartile ranges. P values were determined according to the Mann-Whitney test (*, p<0.05; **, p<0.01).