FIGURE 1 Differentially expressed genes in severe patients with
Class II mutation. Significance was analyzed with unpaired Student
t-test. **: p≤0.01; *: p≤0.05. (A) Up regulated genes (TNFRSF11A; 4,03
FC, KCNE1; 3,90 FC, STX1A; 3,03 FC and SLC9A3R2; 2,02 FC). (B) Down
regulated genes (CXCL1; -5,03 FC, CFTR; -2,92 FC and CXCL2; -2,65 FC).
Among the KEGG pathways that were enriched in WebGestalt analysis, IL-17
signaling pathway, NF-kappa B signaling pathway, cytokine-cytokine
receptor interaction, TNF signaling pathway, NOD-like receptor signaling
pathway, chemokine signaling pathway, SNARE interactions in vesicular
transport and aldosterone regulated sodium reabsorption were the most
prominent ones which could be important in CF severity due to their
roles in inflammation. Pathways and associated genes were summarized in
Table 2. Possible mechanism of inflammation related pathways in severe
patients with Class II mutation was illustrated in Figure 2.
TABLE 2 The list of pathways and differentially expressed genes
in severe patients with Class II mutation (Significantly expressed genes
are shown in bold).