FIGURE 1 Differentially expressed genes in severe patients with Class II mutation. Significance was analyzed with unpaired Student t-test. **: p≤0.01; *: p≤0.05. (A) Up regulated genes (TNFRSF11A; 4,03 FC, KCNE1; 3,90 FC, STX1A; 3,03 FC and SLC9A3R2; 2,02 FC). (B) Down regulated genes (CXCL1; -5,03 FC, CFTR; -2,92 FC and CXCL2; -2,65 FC).
Among the KEGG pathways that were enriched in WebGestalt analysis, IL-17 signaling pathway, NF-kappa B signaling pathway, cytokine-cytokine receptor interaction, TNF signaling pathway, NOD-like receptor signaling pathway, chemokine signaling pathway, SNARE interactions in vesicular transport and aldosterone regulated sodium reabsorption were the most prominent ones which could be important in CF severity due to their roles in inflammation. Pathways and associated genes were summarized in Table 2. Possible mechanism of inflammation related pathways in severe patients with Class II mutation was illustrated in Figure 2.
TABLE 2 The list of pathways and differentially expressed genes in severe patients with Class II mutation (Significantly expressed genes are shown in bold).