Detection of
CD4+CCR6+CRTH2+memory Th2 cells
Blood (100 μl), with heparin as anticoagulant, was mixed with PE-labeled
anti-CRTH2, PE-Cy5.5-labeled anti-CD4 and PE-Cy7-labeled anti-CCR6
monoclonal antibodies and incubated at room temperature avoiding light
for 30 minutes. Red blood cells were removed by lysis, and
CD4+CCR6+CRTH2+memory Th2 cells were detected by CytoFLEX flow cytometry. Data analysis
was performed using CytExpert version 2.3.0.84.