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Characterization and virulence factors distribution of blaCTX-M and mcr-1 carrying Escherichia coli isolates from bovine mastitis
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  • Muhammad Shafiq,
  • Jinhu Huang,
  • Jan Shah,
  • Xiaoming Wang,
  • Sadeeq Rahman,
  • Ilyas Ali,
  • Chen Li
Muhammad Shafiq
Nanjing Agricultural University - Weigang Campus
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Jinhu Huang
Nanjing Agricultural University - Weigang Campus
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Jan Shah
Nanjing Agricultural University - Weigang Campus
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Xiaoming Wang
Nanjing Agricultural University - Weigang Campus
Sadeeq Rahman
Abdul Wali Khan University Mardan
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Ilyas Ali
Nanjing Agricultural University - Weigang Campus
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Chen Li
Nanjing Agricultural University - Weigang Campus
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Abstract

Aim: To investigate the occurrence of ESBL and colistin-resistant E. coli and its genotypic characterization and identification of virulence determinants in cases of bovine mastitis in three provinces of China.
Materials & methods: Five hundred and thirty-five milk samples presenting mastitis symptoms were screened for the presence of ESBL, colistin-resistant, and different virulence genes. Susceptibility testing was identified by the micro-dilution method. E. coli isolates were used to detect ESBL genes (blaCTX-M, blaSHV, and blaTEM) and colistin-resistant genes mcr-(1-9). Multiplex PCR approach was used for the detection of major blaCTX-M groups, different phylogroups, and virulence genes. The clonal relationship was then evaluated with MLST, MLSA, and PFGE.
Results: Multi-drug resistance (MDR) was found in more than 85% of ESBL and colistin-resistant E. coli isolates. Genotypic characterization showed a dominance of the blaCTX-M-1 group, and the most prevalent alleles observed were blaCTX-M-28 (38.37%), blaCTX-M-14 (17.44%), blaCTX-M-66 (13.95%), and blaCTX-M-55 (10.46%). The targeted virulence genes were detected in 97.89% of isolates. Sequence types ST58 and ST410 were the most predominant (2/20=20%). The majority of the E. coli isolates carrying ESBL and mcr-1 were clonally unrelated.
Conclusion: High level of association was observed between ESBL-producing and COL-resistance in E. coli of bovine mastitis.