Analysis of fetomaternal hemorrhage
Determination of fetomaternal hemorrhage (FMH) was done by flow cytometry at time of delivery, using FITC-conjugated Anti-D reagent (9433FI, NHS Blood and Transplant) according to the Guidelines on the estimation of Fetomaternal Hemorrhage.17 The LOD was 0.05% of fetal erythrocytes, corresponding to 1 mL FMH.