2.3 Virus
FMDV serotype A/Arg/2001 (A2001) inactivated (iFMDV) with binary ethyleneimine (provided by Biogenesis Bagó, Buenos Aires) and purified by sucrose gradient was used as a positive control for protein characterization assays, to formulate experimental vaccines, in ELISA and in cellular assays. Infectious virus, obtained from vesicles of experimentally infected cattle (provided by the Argentine National Service of Animal Health, SENASA) with one or two passages in BHK-21 cells, was used for viral challenge. All experiments involving infectious virus were performed in the INTA BSL-4 OIE facilities.