2.1 Animals
All experiments involving the use of animals were carried out according
to INTA Ethics Manual “Guide for the use and care of experimental
animals”, under protocol Number: 26/2016.
Male BALB/c mice, 8-12 weeks old from the animal facilities of the
School of Veterinary Sciences, University of Buenos Aires, Argentina,
were used.
FMDV-seronegative calves as determined by enzyme-linked immunosorbent
assay (ELISA), according to Hamblin et al. (1986), of approximately 8-10
months old, were used in the experiment (Hamblin et al., 1986).