2.3 Virus
FMDV serotype A/Arg/2001 (A2001) inactivated (iFMDV) with binary
ethyleneimine (provided by Biogenesis Bagó, Buenos Aires) and purified
by sucrose gradient was used as a positive control for protein
characterization assays, to formulate experimental vaccines, in ELISA
and in cellular assays. Infectious virus, obtained from vesicles of
experimentally infected cattle (provided by the Argentine National
Service of Animal Health, SENASA) with one or two passages in BHK-21
cells, was used for viral challenge. All experiments involving
infectious virus were performed in the INTA BSL-4 OIE facilities.