Figure 4. The effects of eDNA particle size, water temperature, and target gene on eDNA decay rate constants. Upper and lower graphs show the results for mitochondrial (bright grey) and nuclear (dark grey) eDNA, respectively. Medians and 95% CI of eDNA decay rate constants are indicated by circles and bars, respectively. Each filter pore size (10, 3, 0.8, and 0.2 µm) corresponded to a size fraction (>10, 3-10, 0.8-3, and 0.2-0.8 µm).