Figure 1 Generation and characterization of BMDCs from mice. BMDCs were generated from mouse femurs and tibiae. BMDCs were cultured in 1640 medium with 10% FCS and 20 ng/mL GM-CSF for 9 days. After incubation with PBS, 50 ng/mL IL-10 or 10 ng/mL LPS for 24h, cells were stimulated with 1 μM OVA for 2h, and DCia, DC10 and DClps were generated. CD80, CD11c and MHCII triple -positive cells were identified as BMDCs (lower panel). Noninduced BM cells were triple negative for these markers (upper panel). (A) Schematic of BMDC culture. (B) Characterization of BMDCs by flow cytometry. One representative dot plot is shown.