Adoptive transfer of LPS-treated BMDCs alleviated airway inflammation in OVA-induced asthmatic mice
We used OVA sensitization/challenge to establish an asthma mouse model (Fig. 3A). In this experiment, BMDCs were generated in vitro and used to treat asthmatic mice, as noted. After OVA stimulation, we found that the lung tissue of asthmatic mice exhibited typical inflammatory changes, which were characterized by airway wall thickening, and inflammatory cell infiltration (Fig. 3B). DClps treatment significantly improved the lung inflammation, reduced the lung injury score (Fig. 3C), as well as dramatically diminished the number of inflammatory cells in BALF (Fig. 3D). The analysis of cellular composition of BALF showed DClps remarkably decreased the number of neutrophils and increased the number of lymphocytes (Fig. 3D), suggesting that the adoptive transfer of DClps may be involved in attenuating lung inflammation in asthmatic mice.