Adoptive transfer of LPS-treated BMDCs did not alter the number of pulmonary Tregs
Recent studies have demonstrated that DC10 treatment is associated with the conversion of Th2 effector T (Teff) cells into CD4+CD25+ Foxp3+ Tregs in the lungs of treated mice, and these Tregs are probably involved in inflammation regulation24. Hence, we sought to examine whether DClps treatment increased the number of pulmonary Tregs in a mouse model of asthma. We isolated T cells from the lungs of asthmatic mice on day 19 and evaluated their expression of certain markers (CD4, CD25, and Foxp3) by FACS. Furthermore, in line with previous studies that the induction of tolerance did not change the number of CD4+CD25+Foxp3+ cells in the lungs in asthma models but rather resulted in stronger Tregs activity, we saw negligible variations in the number of Tregs in treated mice (Fig. 7B). Therefore, our results suggest that neither DC10 nor DClps treatment altered the number of lung CD4+CD25+Foxp3+ Tregs.