Adoptive transfer of LPS-treated BMDCs alleviated airway
inflammation in OVA-induced asthmatic mice
We used OVA sensitization/challenge to establish an asthma mouse model
(Fig. 3A). In this experiment, BMDCs were generated in vitro and used to
treat asthmatic mice, as noted. After OVA stimulation, we found that the
lung tissue of asthmatic mice exhibited typical inflammatory changes,
which were characterized by airway wall thickening, and inflammatory
cell infiltration (Fig. 3B). DClps treatment significantly improved the
lung inflammation, reduced the lung injury score (Fig. 3C), as well as
dramatically diminished the number of inflammatory cells in BALF (Fig.
3D). The analysis of cellular composition of BALF showed DClps
remarkably decreased the number of neutrophils and increased the number
of lymphocytes (Fig. 3D), suggesting that the adoptive transfer of DClps
may be involved in attenuating lung inflammation in asthmatic mice.