Figure 4 Adoptive transfer of LPS-BMDCs to OVA-induced asthmatic mice reduced the number of pulmonary memory CD4+ T cells. OVA sensitization and challenge was similar to that in the previous schematic. BMDCs were adoptively transferred to OVA-sensitive mice for 3 consecutive days. Rechallenge with OVA was performed for 5 consecutive days starting on day 35. Mice were sacrificed on day 40. Central memory CD4+ T cells were CD62L and CCR7 double positive by flow cytometry. Effector memory CD4+ T cells were CD62L and CCR7 double negative by flow cytometry. The proportion of the two subsets of memory CD4+ T cells was not significantly different among the DCia, DC10 or DClps groups. (A) Schematic of the OVA-induced asthmatic mouse model. (B) Analysis of CD4+CD44high memory T cells by flow cytometry.