Introduction: Pharmacist’s knowledge about different aspects of this pandemic is crucial because it influence their role and contribution as a frontline health care provider, as pharmacies and most of the pharmacy practice sectors are kept open even during lockdowns providing counseling, patient care. Pharmacist can provide valuable services during COVID-19 pandemic, these services may include: provide reliable information on the disease, participate in public education on preventive measures, referring of suspected cases, insuring continuous supply of medicine. Methods: A web-based, cross-sectional study, conducted using survey instrument to obtain responses from Sudanese pharmacists during the period from 26th of May to 3rd of June 2020. A 14-item survey instrument was developed. The web-based cross-sectional study was carried out among Sudanese pharmacists. A self-reported structured questionnaire was divided into three sections: demographic characteristics, questions assessing the knowledge, and one question for the pharmacist contribution during the pandemic. Results: The study showed that 51.1% of pharmacists have good knowledge about the COVID-19. The work experience and education level significantly (P<0.05) influence pharmacist knowledge. Majority of pharmacists contribute to different activities during the pandemic, e.g. providing patients with transmission information (94%), Provide factual and reliable information on the diseases symptoms (93.1%), providing patients with prevention information (91.1%). Conclusion: The study identified that pharmacists have good knowledge about COVID-19 pandemic Also pharmacists contributed in many activities as a frontline health care provider during this pandemic.