Figure 2. Duck embryos died during the incubation and the pathological changes in Embryo and ducklings.
(A) The eggs of the experimental group vary in size; (B) The number of inconsistent shape eggs and sand-shelled eggs increased; (C) Unfertilized egg in infected group; (D) Some duck embryos had difficulty in breaking shells at the later stage of hatching; (E) Severe bleeding of embryo body; (F) Dead duck embryo with hemorrhage in liver, (1) Infected group duck embryo and (2) control duck embryo; (G) Some ducklings were depressed; (H) Spleen of duckling presents with severe splenic necrosis.
Figure 3. CPE in N-DRV-SD19 infected LMH cells. (A) The second generation of LMH cells showed CPEs 24 hr after infection; (B) The second generation of LMH cells showed CPEs 72 hr after infection; (C) Non-infected LMH cell 24 hr after test; (D) Non-infected LMH cell 72 hr after test.
Figure 4. Phylogenetic tree constructed based on nucleotide sequences of S1 genome segment by Orthoreovirus species.