Assessment of barcoding success
One of the factors determining a successful COI bacterial
amplification is the initial failure of an extract to amplify mtDNA.
Subsequently, to determine the likelihood of this event within taxa, we
used the 55,366 specimen representative data subset to evaluate failure
rates. To this end, all orders of host which gave at least one
non-target Rickettsia COI hit were assessed. The barcoding
success rate was determined as the proportion of specimens which matched
initial morphotaxa assignment and were not removed after BOLD quality
control
(Ratnasingham &
Hebert, 2007). As the total Rickettsia count was from a larger
dataset than the one made available, an adjusted prevalence for each
taxon was calculated based on the representative data subset.
Targeted and bioinformatic Rickettsia screens