Whole exome sequencing
Genomic DNA samples were extracted from chorionic villi, amniocytes,
fetal tissues or parental blood using a Qiagen DNA Blood Midi/Mini kit
(Qiagen GmbH, Hilden, Germany) following the manufacturer’s protocol.
Then WES was performed according to the published
paper5. And data was analyzed on the Cruxome system
developed by Berry Genomics.