Rubisco activation state assay
N. benthamiana leaves were incubated at 400 ppm
CO2 until they reached steady state photosynthesis, then
the CO2 was switched to 1500 ppm for a specified time.
The plants were then sampled by freeze-clamp (Schrader, Wise, Wacholtz,
Ort & Sharkey 2004). Rubisco activation state was assayed according to
Li et al. (2019).