3.3 Intra-PrL or IL injection of 1-MT blocks depressive
behaviors induced by ICV-STZ.
To directly evaluate whether the activation of IDO in the PrL and IL was
responsible for the depressive- and anxiety-like behaviors in ICV-STZ
rats, we observed the effects of IDO inhibitor 1-MT on the behaviors of
ICV-STZ rats. Depressive-like behaviors induced by ICV-STZ, including
the increase in immobility time in the FST( F(5,30)=34.96, Figure 4A), the decrease in
sucrose preference rate in the SPT( F(5,30)=11.37, Figure 4B) and latency time to
feed in the NSFT ( F(5,30)=16.20, Figure 4C)
were reversed by intra-PrL or IL injection of 1-MT. Food consumption
after NSFT was no difference across the groups (Figure 4D). The result
of time in center showed that intra-PrL or IL injection of 1-MT could
not reverse the anxiety-like behavior in ICV-STZ rats (Figure 4E).
Results of locomotor activity in total distance in the OFT did not
reveal significant differences among the various groups ( Figure
4F, G). Based on these results, intra-PrL or IL injection of 1-MT both
prevented depressive-like behaviors in ICV-STZ rats but failed to
produce an anxiolytic effect.