Novelty-suppressed feeding test
The NSFT is most widely used to check the efficacy and efficiency of chronic and sub-chronic antidepressant treatments in rodent models(Blasco-Serra, Gonzalez-Soler et al., 2017). The test was performed in an open field containing five to six food pellets placed in the middle of the arena. Rats were deprived of food at least 24 h before the test. After SPT, individual rats were placed in the corner of the arena and allowed to explore it freely for 10 minutes. The latency for the rat from leaving the corner of the arena to pick up food was recorded. The latency to feed was measured up to 10 minutes. The increase in latency to feed is considered a measure of anhedonia based on food. After the NSFT, the rats were returned to their home cages and allowed to eat food. Food consumption within 60 minutes across the groups was recorded to exclude the non-specific ingestive behavior.