2.5 Experiment design and behavioral assessment
Animals with surgery-related infections were excluded. Then, the rats were randomly grouped into six groups, with six animals (n=6) in each group presented in Figure 1A. The cannula placements of the lateral ventricle are shown in Figure 1B. Animal body weight and food consumption were measured daily. The water intake was measured daily from day 1 to day 5 before food and water deprivation. All behavioral tests except the novel object recognition test were conducted on day 7 and performed as previously described(Ding, Cui et al., 2021; Ye, Cui et al., 2018). On day 7, the open field test was performed at 9:00 AM, the sucrose preference test was performed at 12:00 PM, the novelty-suppressed feeding test was performed at 2:00 PM, and the forced swim test was performed at 4:00 PM. The novel object recognition test was performed at 9:00 AM on day 8. The rats were decapitated immediately after the behavioral tests on day 8, and the tissue was used for western blot and HPLC‒MS/MS. The other three same experiments with six animals (n=6) in each group were used for ELISA, immunofluorescence staining and Golgi staining.