2.11 Data and Statistical analysis
Data and statistical analysis complied with the recommendations of the
BJP on experimental design and analysis in pharmacology(Curtis,
Alexander et al., 2018). The sample size was the number of independent
values, and statistical analysis was performed using these independent
values. The sample sizes are indicated in the figure legends. The data
from western blot were normalized to control group values and determined
as ’Fold change’ in figures. Statistical analysis of the results was
done with the help of GraphPad Prism software (version 8; GraphPad
Software, Inc., RRID: SCR_002798). Statistical analysis was undertaken
only for studies where each group size was at least n=6. The data are
presented as the means ± SEM. Unpaired t-test was used to test data for
vehicle and ICV-STZ model of variance. One-way and two-way analysis of
variance (ANOVA) followed by Tukey’s multiple comparison test was
applied for comparison among several groups. For two-way ANOVA, the
procedure (vehicle or ICV-STZ) and the treatment (saline or 1-MT) were
taken as between-group factors. P<0.05 was considered
statistically significant in all tests. The experimenter or observer was
blind to the groups while analysing the results.
3 Result s