Experimental approach
IDO inhibitor 1-MT was directly injected into PrL and IL of depressive
rats induced by ICV-STZ, respectively. Depressive- and anxiety-behaviors
were evaluated in forced swim test, sucrose preference test,
novelty-suppressed feeding test, novel object recognition test and open
field test. HPLC‒MS/MS detected kynurenine metabolites, ELISA detected
cytokines, Western blot examined protein expression and Golgi staining
assessed synaptic plasticity. Immunofluorescence staining was used to
observe the expression and morphology of glial cells.