Open field test
The OFT was performed to evaluate locomotor activity and anxiety-like
behavior. Rats were submitted individually to a Plexiglas chamber (40
cm×40 cm×65 cm), and behavior was recorded by an automated video
tracking system (DigBehv-LM4, Shanghai Jiliang Software Technology,
Shanghai, China). The video files were later analysed using DigBehv
analysis software. In 10 minutes, locomotor activity is expressed as the
total distance, and time in the center is considered an indicator of
anxiety-behavior. The apparatus was wiped with 75% alcohol between
tests to eliminate any smell.