2.11 Data and Statistical analysis
Data and statistical analysis complied with the recommendations of the BJP on experimental design and analysis in pharmacology(Curtis, Alexander et al., 2018). The sample size was the number of independent values, and statistical analysis was performed using these independent values. The sample sizes are indicated in the figure legends. The data from western blot were normalized to control group values and determined as ’Fold change’ in figures. Statistical analysis of the results was done with the help of GraphPad Prism software (version 8; GraphPad Software, Inc., RRID: SCR_002798). Statistical analysis was undertaken only for studies where each group size was at least n=6. The data are presented as the means ± SEM. Unpaired t-test was used to test data for vehicle and ICV-STZ model of variance. One-way and two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test was applied for comparison among several groups. For two-way ANOVA, the procedure (vehicle or ICV-STZ) and the treatment (saline or 1-MT) were taken as between-group factors. P<0.05 was considered statistically significant in all tests. The experimenter or observer was blind to the groups while analysing the results.
3 Result s