Open field test
The OFT was performed to evaluate locomotor activity and anxiety-like behavior. Rats were submitted individually to a Plexiglas chamber (40 cm×40 cm×65 cm), and behavior was recorded by an automated video tracking system (DigBehv-LM4, Shanghai Jiliang Software Technology, Shanghai, China). The video files were later analysed using DigBehv analysis software. In 10 minutes, locomotor activity is expressed as the total distance, and time in the center is considered an indicator of anxiety-behavior. The apparatus was wiped with 75% alcohol between tests to eliminate any smell.