Laboratory analysis
The tissue samples were homogenized and total genomic DNA was extracted
from tissue and blood samples using QIAamp DNA mini kit (Qiagen, Hilden,
Germany) following the manufacturer’s instructions. ASF diagnosis was
carried out by detection of ASFV genome using conventional polymerase
chain reaction (PCR) using primers PPA1: 5’-AGT TAT GGG AAA CCC GAC
CC-3’ and PPA2 5’-CCC TGA ATC GGA GCA TCC T-3’, targeting theB646L gene of the ASFV as previously described (Agüero et al.,
2003). Amplified PCR products were separated and visualized by gel
electrophoresis, with 257 bp being the expected PCR product