2.3 Sample collection and 16S ribosomal RNA (rRNA)
high-throughput sequencing
Cecum and jejunum contents were quickly collected in 5 ml cryotubes
following slaughter in each case. Intestinal contents samples were
collected in five tubes and quickly placed in liquid nitrogen to be
stored for subsequent intestinal 16S rRNA microbial detection and
analysis. Intestinal microbial DNA extraction, detection, PCR
amplification, and product purification from different intestinal
samples was carried out before 16S rRNA gene sequencing was performed at
Nuohe Biotechnology Co., Ltd.