Supplementary results
Supplementary Figure S1. Western blot representative bands of
several markers involved in distinct pathways of apoptosis. (A)
Extrinsic pathway, (B) intrinsic pathway and (C) Reticulum endoplasmic
mediated pathway. (D) Scheme of the general mechanism of action of
tigecycline in apoptosis induction. Data are presented as mean ± SEM.
Supplementary Figure S2. Effect of tigecycline and 5-FU on
murine CAC model. (A) Survival rates. (B) Tumor volume. Representative
Western blot bands of the different markers involved in (C)
proliferation, (D) epithelial-mesenchymal transition and (E) apoptosis.
Supplementary Figure S3. Flow cytometry analysis of myeloid
cells populations . (A) Gating strategy for the selection of different
populations of immune cells and (B) percentage of each one. Data are
presented as mean ± SEM. Bars with different letter differ statistically
(P<0.05).
Supplementary Figure S4. Flow cytometry analysis of lymphoid
cells populations. (A) Gating strategy for the selection of different
populations of immune cells and (B) percentage of CD3+ and regulatory T
cells. Data are presented as mean ± SEM. Bars with different letter
differ statistically (P<0.05).