Figure headings
Figure 1: Areas where soil was collected in northern Germany. Sources of
yard soil, swamp mud, beach sand, and two forest soils are shown.
Figure 2: Downstream protocol for processing of soil samples for virus
isolation and qPCR. Pictures show sample after media and soil matrix has
been sonicated and centrifuged for 30 minutes at 2,500 x g at 4°C.
Figure 3: Infectious wild boar blood (7.25 log10HAD50/mL of ASFV Armenia08) and yard soil spiked with
400μL of it was stored at 25°C or 4°C. ASFV genome copies per mL are
depicted in black and virus titer is shown in red. Experiments were
completed in triplicates and each circle represents each individual
replicate.
Figure 4: (A) Different soil types spiked with 1.2 mL infectious blood
(6.0 log10 HAD50/ml of ASFV Armenia08)
and stored at room temperature. Genome copies are depicted in black and
virus titer is shown in red. (B) Beach sand and sterile sand were
inoculated with 2 mL of infectious blood and also incubated at room
temperature. Blood only samples served as controls in both experiments.
ASFV genome copies are depicted in black and virus titers are shown in
red.
Figure 5: Cytopathic effect and simple ASFV titration readout with WSL
cells infected with ASFV KenyaΔCD2v-dsRed.
Figure 6: Different soil types were spiked with 2 ml of spiked blood
(6.0 log10 HAD50/mL of ASFV KenyaΔCD2v-dsRed) and stored at room
temperature for indicated times. ASFV genome copies (A) and virus titers
on WSL cells (B) in untreated or disinfectant-treated matrices are
shown.