2.4. Enzyme-Linked Immunosorbent Assay (ELISA)
For intestinal cultures, 1 cm piece of tissue was cut from the ileum cleaned with PBS containing antibiotics 5 times. The pieces were cultured in a 5% CO2 incubator at 37 ºC for 48 hours containing anti-anti. Supernatants were collected for ELISA. Blood from mice was centrifuged and serum was then frozen at -80 ° C. Supernatants were used for mouse IL-22, IL-17A and GM-CSF ELISA. The manufacturer’s protocol was followed.