Unsal Savci

and 14 more

Objectives: Myroides spp. is an environmental pathogen and causes disease in immunocompromised patients. In this study, we report an outbreak of urinary tract infections caused by M. odoratimimus in a university hospital in Turkey. Methods: A total of 25 M. odoratimimus strains isolated from the clinical samples of 20 patients in our intensive care units and clinics were included in the study. Phenotypic and genotypic identification of isolates was performed using conventional methods, VITEK®-2 automated identification system, Matrix Assisted Laser Desorption/Ionisation-Time of Flight Mass Spectrometry and 16S-RNA Microbial Diagnosis methods. In addition, Repetitive Extragenic Palindromic Elements (REP) PCR Assay method was applied for molecular epidemiological analysis. Results: All cases were diagnosed with nosocomial urinary tract infection, except for one case diagnosed with nosocomial bacteraemia. One of the M. odoratimimus isolates was sensitive to piperacillin/tazobactam (MIC: ≤4 µg/ml) and one isolate was moderately sensitive to cefepime (MIC: 16 µg/ml). Other M. odoratimimus isolates were resistant to the tested antibiotics of beta lactams, monobactams, carbapenems, aminoglycosides, fluoroquinolones and sulphonamides. When 10 isolates were evaluated with the REP PCR method, DNA finger print similarities were visually determined and there was a similar DNA pattern among them. Myroides source was not detected in environmental samples. Conclusion: Clinicians should consider that Myroides spp. isolates with multiple and broad-spectrum drug resistance may be a serious nosocomial pathogen like Pseudomonas aeruginosa or Acinetobacter baumannii. In order to choose the best treatment regimen, this atypical pathogen needs to be quickly identified and antibiotic susceptibility tests performed.

Coskun Kaya

and 3 more

Aims: To evaluate the diagnostic significance of the novel index combining preoperative hemoglobin, albumin levels, lymphocyte and platelet counts (HALP) in prostate cancer (PCa) patients. Methods: Between January 1, 2015 to December 31, 2018 at the Hitit University Erol Olçok Education and Research Hospital 225 patients who had undergone transrectal ultrasound guided prostate biopsy or prostate surgery were analyzed retrospectively. A total of patients; 155 had benign prostate hyperplasia (group 1) and 70 had PCa (group 2). The preoperative serum levels of hemoglobin, albumin, lymphocyte counts, and platelet counts were recorded. The HALP scores and the sub parameters of this index for each of the two groups were compared. Results: The total Prostate Specific Antigen (PSA), albumin and lymphocyte differences between the groups were statistically significant (p=0.0002, p=0.0001, p= 0.005). The median value of HALP scores in Group 1 and 2 were 49.43 and 51.2 respectively, and this was not statistically significant between groups (p=0.737). The HALP score had the least Area Under Curve (AUC) value compared to the others (0.514). the AUC of Albumin was larger than PSA for diagnostic efficacy in PCa patients (0.696-0.656). However, albumin levels were statistically significant compared to platelet count and the HALP score (p=0.0033, p=0.0068), except PSA and lymphocyte (p=0.4580, p=0.1717). Conclusion:Further prospective clinical studies that include more patients from multiple centers are needed to show the diagnostic role of the HALP score and its compounds on the patients with PCa.