Preparation of genomic DNA (gDNA) and complementary DNA (cDNA)
gDNA was prepared from 2 × 106 cells using Solg™
genomic DNA prep kit (SGD41-C100; Solgent, Daejon, Korea) according to
the manufacturer’s instructions. mRNA was isolated from
2 × 106 cells using Rnasy Mini Kit (74104; QIAGEN,
Hilden, Germany). mRNA was reverse-transcribed using phusion RT-PCR Kit
(F-546S; Thermo Fisher Scientific) according to the manufacturer’s
instructions. Purity and concentration of the gDNA and cDNA were
determined using a DS-11 Spectrophotometer (DeNovix, Wilmington, DE,
USA).