Quantitative PCR (qPCR)
qPCR was performed using a CFX Connect™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) using a Solg™ 2× real-time PCR smart mix (SRH83-M40h; Solgent). RT-qPCR was performed either with 20 ng of gDNA or cDNA, with denaturation at 95°C for 5 min followed by 40 cycles of 94°C for 30 s, 57°C for 30 s, and 70°C for 10 s.