Preparation of genomic DNA (gDNA) and complementary DNA (cDNA)
gDNA was prepared from 2 × 106 cells using Solg™ genomic DNA prep kit (SGD41-C100; Solgent, Daejon, Korea) according to the manufacturer’s instructions. mRNA was isolated from 2 × 106 cells using Rnasy Mini Kit (74104; QIAGEN, Hilden, Germany). mRNA was reverse-transcribed using phusion RT-PCR Kit (F-546S; Thermo Fisher Scientific) according to the manufacturer’s instructions. Purity and concentration of the gDNA and cDNA were determined using a DS-11 Spectrophotometer (DeNovix, Wilmington, DE, USA).