Nested PCR and sequencing
All primers were designed using the Snap Gene software (GSL Biotech LLC,
Chicago, IL, USA) and synthesized by Macrogen (Seoul, Korea). Nested PCR
was performed using a thermal cycler (9700; Perkin Elmer, Waltham, MA,
USA) using Solg™ 2×Taq PCR smart mix 2 (STD02-M50h; Solgent). Nested PCR
was performed with 20 ng of gDNA, with denaturation at 95°C for 5 min
followed by 40 cycles of 94°C for 30 s, 57°C for 30 s, and 70°C for 10
s. PCR fragments were purified using Solg™ gel and PCR purification
system (SGP04-C200; Solgent) and sequenced by Macrogen.