Combination of ALX and FSK inhibited GRK5 expression and its nuclear activities in PMɸ
Nuclear translocation of immune cells GRK5 in PMɸ was investigated with immunofluorescence (IF). After treatment of PMɸ-LPS-ISO with ALX, FSK, and their combination overtime, IF staining for GRK5 (R-PE dye), nuclei (DAPI), and cytoplasmic membrane (CTxB - Alexa Fluor® 488 Dye) was done. Imaging showed that without any therapeutic interventions, GRK5 was upregulated in PMɸ-LPS-ISO. ALX single therapy inhibited GRK5 expression significantly and blocked its nuclear translocation activities. Meanwhile, the FSK single treatment had an insignificant effect on GRK5 expression and its nuclear translocation activities. As such, the FSK and ALX combination did just as excellent as the ALX single therapy (Fig. 4).