Combination of ALX and FSK attenuated CCS-induced PCH
Morphometric assessment of whole hearts showed that PCH mice had an
increase in their heart size as by their heart weight (HW) and body
weight (BW) ratio. Comparing the therapeutic groups with the Vhl and PCH
groups, ALX alone and its combination with FSK did better at keeping the
HW/BW ratio healthy than FSK single therapy (Fig. 8a and Supplementary
Table S2). Detailed morphological analysis of longitudinal and
transverse tissue sectionings using H&E and trichrome staining revealed
hearts PCH mice had dilated left and right heart chambers, marked
collagen deposits, and abnormal hypertrophied cardiomyocytes (Fig.
8a-8c). Again, although ALX effectively attenuated cardiomyocyte
hypertrophy, it failed to prevent interstitial collagen deposits. Also,
FSK alone was not very efficient in attenuating neither myocyte
hypertrophy, nor collagen deposit as its combination with ALX did (Fig.
8d and 8e).