Combination of ALX and FSK minimized immune cells infiltration
into myocardium and systemic inflammatory response
Ascertaining the extent of mononuclear immune cells infiltrating the
myocardium in response to cardiomyocyte necrosis was done CD68 IHC
staining. As expected, PCH mice an enormous infiltration immune cell.
Comparatively, the FSK single therapy decreased the infiltration of
immune cells than the ALX single therapy did. Nonetheless, their
combination was effective in minimizing macrophages and other
mononuclear cell infiltration into the myocardium (Fig. 9a).
Inflammatory cytokines expression evaluated with ELISA illustrated that
among the PCH-preventive therapeutic groups, the FSK and ALX combination
therapy had the most efficacy in maintaining a close homeostatic gap
between proinflammatory and anti-inflammatory responses during CCS. The
secretions of IL-1β, IL-6, and TNFα by macrophages in response to
necrotic cardiomyocytes were moderate in the mice treated with the
combination therapy (Fig. 9b-9d), while IL-10 was upregulated in these
mice (Fig. 9e). Treatment with only FSK did better than the treatment
with only ALX in the attempt to prevent a hyperactive immune response.