Combination of ALX and FSK inhibited GRK5 expression and its
nuclear activities in PMɸ
Nuclear translocation of immune cells GRK5 in PMɸ was
investigated with immunofluorescence (IF). After treatment of
PMɸ-LPS-ISO with ALX, FSK, and their combination
overtime, IF staining for GRK5 (R-PE dye), nuclei (DAPI), and
cytoplasmic membrane (CTxB - Alexa Fluor® 488 Dye) was done. Imaging
showed that without any therapeutic interventions, GRK5 was upregulated
in PMɸ-LPS-ISO. ALX single therapy inhibited GRK5
expression significantly and blocked its nuclear translocation
activities. Meanwhile, the FSK single treatment had an insignificant
effect on GRK5 expression and its nuclear translocation activities. As
such, the FSK and ALX combination did just as excellent as the ALX
single therapy (Fig. 4).