Combination of ALX and FSK minimized LPS-induced inflammatory
response during CCS with much efficacy
The inhibitory effect of ALX on GRK5, which participates in maladaptive
activities in cardiac and immune cells, was used to treatment
PMɸ challenged with LPS under CCS condition to attenuate
the invocation of a hyperactive immune response. FSK was also utilized
to directly stimulate ACs to synthesize cAMP, which has a positive
effect on chronotropic and inotropic in cardiomyocytes and an
anti-inflammatory effect in immune cells to modulate PMɸresponse during CCS. Also, the stressed PMɸ challenged
with LPS were also treated with the combination of ALX and FSK.
Results from ELISA analysis of the supernatants from all groups are
demonstrated in (Fig. 3). Although all the therapeutic invention groups
(ALX, FSK, and ALX with FSK) decreased the extent of immune
hyperactivation of PMɸ elicited by LPS during CCS, the
single treatment of ALX had the least efficacy compared with FSK single
therapy and their combine therapy. More than the single treatment with
FSK after the PMɸ had been challenged with LPS under
stress, the combination treatment with ALX and FSK was effective in
minimizing IL-1β, IL-6, and TNFα (Fig. 3a-3c) secretions while keeping
IL-10 upregulated (Fig. 3d).