Sequence analysis
Online similarity searches were performed using the BLAST algorithm of GenBank. Sequence alignment was carried out with the CLUSTALW program by MEGA7 software(S. Kumar, Stecher, & Tamura, 2016). The phylogenetic analyses based on the 16S rDNA gene for proteolytic bacteria were further used to establish relationships among them. The partial 16S rDNA sequences obtained for were utilized in the search of reference nucleotide sequence available in NCBI GenBank database using BlastN algorithm to retrieve the similar sequences(Altschul et al., 1997). Phylogenetic tree and evolutionary relationships of bacterial isolates was constructed by Neighbor-Joining method (Saitou & Nei, 1987) with Kimura 2-parameter corrections (Kimura, 1980). To calculate the support for each clade, bootstrap analysis were performed with 1000 replications (Felsenstein, 1985).