Data analysis
Metabolite identification was done by MetabolitePilotTM Software version 2.0 (SCIEX). The MS/MS spectrum of IVM was exported as text files by the PeakView software (SCIEX) and imported to MetabolitePilotTM software (SCIEX) as a reference spectrum for creating the ivermectin library. Raw data files (.wiff) of metabolite sample analyses were imported to MetabolitePilot TMsoftware and compared against the IVM-library peak finding strategies as described in the supplementary material. For the LC-SPE-NMR/MS system, the HPLC was operated by Hystar 3.2 (Bruker Daltonics), mass spectrum acquired by Microtof control (Bruker Daltonics), and the NMR spectrometer was operated by Topspin 3.5 (Bruker Biospin). The Complete Molecular Confidence – Structure Elucidation (CMC-se) software version 2.6.1 (Bruker Biospin,) was used for structure elucidation.