EXPERIMENTAL APPROACH
Human liver microsomes, primary human hepatocytes, and whole blood from
healthy volunteers given oral ivermectin were used to identify
ivermectin metabolites by ultra-high performance liquid chromatography
coupled with high resolution mass spectrometry. The molecular structures
of metabolites were determined by mass spectrometry and verified by
nuclear magnetic resonance. Pure cytochrome P450 enzyme isoforms were
used to elucidate the metabolic pathways.