Materials
Ivermectin (IVM-B1a > 95%,
IVM-B1b < 2%) was purchased from
Sigma-Aldrich (Saint Louis, MO, USA). IVM-B1a (95%
purity) was purchased from Toronto Research Chemical (Toronto, ON,
Canada). IVM-B1b (99.27% purity) was purchased from
Clearsynth (Mississauga, Ontario, Canada). Pooled human liver microsomes
(50 donors, GibcoTM) were purchased from Thermo Fisher
Scientific (Waltham, MA, USA). Cryopreserved primary human hepatocytes
(M00995-P, UBV donor) and hepatocyte culture medium (InVitroGroTM CP
Medium) were purchased from BioIVT, Inc (Baltimore, MD, U.S.A.). Human
cytochrome P450 cDNA-expressed (Supersomes™) isoforms (CYP1A2, CYP2B6,
CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, CYP3A4, and CYP3A5),
NADPH regenerating system, insect cell microsomes (negative control),
and human P450 oxidoreductase +b5 were purchased from Corning (Corning,
NY, USA). LC-MS grade water and acetonitrile were purchased from J.T
Baker (City, PA, USA). LC-MS grade ammonium acetate and formic acid were
purchased from Sigma-Aldrich (Saint Louis, MO, USA). HPLC grade organic
solvent for NMR analysis was purchased from Sigma Aldrich (Saint Louis,
MO, USA). CD3CN 99.8% and CDOOD 98% was purchased from Deutero GmbH
(Kastellaun, Germany). Water for NMR was prepared from a Milli-Q
purification system from Merck (Darmstadt, Germany).