Figure 1. Field sampling and inferred population structure ofP. auritus . (a) Map of nine sampling locations: Takamanda (TM),
Ndikiniméki (ND), Ebo Forest (EF), Campo Ma’an (CM), Monts de Cristal
(MC), Minkébé (MK), Lopé (LP), Kessala (KS) and Gamba Complex (GC). Each
point is a sample site and the colors correspond to the assigned
population. (b) PCA using SNPs that have a minor allele frequency
> 5%. Each point presents a sample, and samples are
colored by site with similar color shades corresponding to their
assigned populations. (c) FastStructure results using SNPs that have a
minor allele frequency > 5% and filtered to have only one
random SNP per locus. Site abbreviations are labeled within each
population.
Figure 2. Morphological variation from RF projections using
morphometric data and environmental variables (a) Variation in body
size, represented by the first principle component of a PCA of all seven
linear morphometric measurements. (b) Variation in relative leg length.
(c) Variation in head shape, represented by the first principle
component of a PCA of geomorphometric head shape calculated in a SHAPE
analysis. For these maps, the greater the color difference, the greater
difference in morphology.
Figure 3. GF results with maps of genomic variation and genomic
vulnerability. (a) PC plot with labelled vectors indicating the
direction and relative magnitude of environmental variables with the
greatest contribution to the predicted patterns of SNP allele frequency
differentiation. (b) Environmental and geographic variables ranked by
their importance in explaining SNP allele frequency variation. (c) Map
of the gradient forest model of environmentally-associated SNPs forP. auritus . Larger color differences between any two areas in the
landscape correspond to larger genetic differences. Circles indicate
sampling sites (d) Genomic vulnerability. Red indicates greater changes
in allele frequencies and thus, higher genomic vulnerability, while blue
indicates smaller changes in allele frequencies and less genomic
vulnerability.