Figure 1. Field sampling and inferred population structure ofP. auritus . (a) Map of nine sampling locations: Takamanda (TM), Ndikiniméki (ND), Ebo Forest (EF), Campo Ma’an (CM), Monts de Cristal (MC), Minkébé (MK), Lopé (LP), Kessala (KS) and Gamba Complex (GC). Each point is a sample site and the colors correspond to the assigned population. (b) PCA using SNPs that have a minor allele frequency > 5%. Each point presents a sample, and samples are colored by site with similar color shades corresponding to their assigned populations. (c) FastStructure results using SNPs that have a minor allele frequency > 5% and filtered to have only one random SNP per locus. Site abbreviations are labeled within each population.
Figure 2. Morphological variation from RF projections using morphometric data and environmental variables (a) Variation in body size, represented by the first principle component of a PCA of all seven linear morphometric measurements. (b) Variation in relative leg length. (c) Variation in head shape, represented by the first principle component of a PCA of geomorphometric head shape calculated in a SHAPE analysis. For these maps, the greater the color difference, the greater difference in morphology.
Figure 3. GF results with maps of genomic variation and genomic vulnerability. (a) PC plot with labelled vectors indicating the direction and relative magnitude of environmental variables with the greatest contribution to the predicted patterns of SNP allele frequency differentiation. (b) Environmental and geographic variables ranked by their importance in explaining SNP allele frequency variation. (c) Map of the gradient forest model of environmentally-associated SNPs forP. auritus . Larger color differences between any two areas in the landscape correspond to larger genetic differences. Circles indicate sampling sites (d) Genomic vulnerability. Red indicates greater changes in allele frequencies and thus, higher genomic vulnerability, while blue indicates smaller changes in allele frequencies and less genomic vulnerability.