4. Fungal and plant material
Trichoderma gamsii T6085 (Tgam ) (Baroncelli et al., 2016;
Matarese et al., 2012) and Fusarium graminearum ITEM 124
(Fgra ) (Zapparata et al., 2017) were grown on PDA (Sigma) plates
at 25℃, 12 h/12 h light/darkness. Seeds from Triticum aestivumcv. Apogee (wheat) were sown in pots in a potting mix and
incubated in a growth chamber with photoperiod 16 h light/8h dark, at
20℃/22℃. Before all experiments, wheat seeds were surface sterilized
with a NaClO solution (0.6% active chlorine) for 3 min on shaking,
followed by three washing steps of 10 min each with sterile distilled
water. Seeds were stored in sterile distilled water at 4℃ for 3 days for
vernalization.