7. Tgam – wheat roots interaction
Four wheat seeds were placed on PDA at 1.5 cm of distance from the
centre of the Petri dish. Plates were sealed with tape and incubated for
24 h at 22℃. After 24 h, 1 cm2 agar plug cut out at
the border of 1-week-old colony of Tgam was placed in the centre
of the plates containing wheat seedlings, as well as in PDA plates
without plants as controls. Plates were sealed with tape and incubated
for 3 days in the same conditions described above. Wheat roots colonized
by Tgam were collected, frozen in liquid N2 and
stored at -80℃ until RNA extraction. Mycelium from Tgam PDA
control plates was collected and stored until use. Three biological
replicates were included for each condition.