Single cell transcriptomics
Single cell transcriptomic data covering various B and T cells, monocytes, granulocytes and dendritic cells were analyzed. “The Blood Atlas’ collects data from the three datasets (HPA (https://www.proteinatlas.org/humanproteome/blood), Monaco et al 10, and Schmiedel et al 11). HPA presents data for 18 cell types present in human blood that were isolated with cell sorting followed by RNA-seq analysis. Annotation of the studied genes was done according to cell type specificity (cell type enriched, group enriched, cell type enhanced and low cell type specificity) and cell type distribution (detected in single, few, many or all cell types).
Further details of the assays and annotation used by the Human Protein Atlas can be accessed at:https://www.proteinatlas.org/about/assays+annotation#ihk.