Comparison of species composition between morphology, bulk DNA
and eDNA datasets
The number of unique and shared species for the morphological, bulk and
ethanol datasets was evaluated using the unrarified datasets from the
four samples that had been identified morphologically and using the
UpsetR package(Conway, Lex, & Gehlenborg, 2017). For the morphological
dataset, only specimens identified up to species level were included in
the dataset (juvenile identifications to genus, order or phylum level
were removed from the dataset). For each of the five primer sets, the
total number of species found for each of the four samples was
determined and visualized in barplots using R.