2.7 Flow cytometric analysis
The tumor cells and BMDMs were pre-treated and stained with
surface-specific antibodies for 30 min in the dark at 4 °C. Following
washes with PBS, they were detected using flow cytometry (BD FACSVerse,
USA). Specific antibodies used were: anti-CD45-BV421, anti-CD11b-FITC,
anti-F4/80-PE, and anti-CD206-APC were purchased from BioLegend (San
Diego, CA, USA). Directly conjugated, isotype-matched, rat anti-mouse
Abs (BioLegend) served as controls.