(BMDMs)
The method for obtaining BMDMs is shown in Fig. 3A. In brief, bone marrow cells were isolated from the femur and tibia of mice (6–8 weeks of age, SPF) with PBS. The cells were then cultured in complete DMEM containing GM-CSF (10 ng/mL, PeproTech, USA) and IL-28B (0, 100, 200, and 400 ng/mL). After four days, the medium was supplemented as described above. On the seventh day, BMDMs were obtained, and IL-4 (10 ng/mL, PeproTech, USA) and IL-13 (10 ng/mL, PeproTech, USA) were used to polarize cells to M2 macrophages for subsequent experiments.