(BMDMs)
The method for obtaining BMDMs is shown in Fig. 3A. In brief, bone
marrow cells were isolated from the femur and tibia of mice (6–8 weeks
of age, SPF) with PBS. The cells were then cultured in complete DMEM
containing GM-CSF (10 ng/mL,
PeproTech, USA) and IL-28B (0, 100, 200, and 400 ng/mL). After four
days, the medium was supplemented as described above. On the seventh
day, BMDMs were obtained, and IL-4 (10 ng/mL, PeproTech, USA) and IL-13
(10 ng/mL, PeproTech, USA) were used to polarize cells to M2 macrophages
for subsequent experiments.