2.5 Cell viability assays
MC38 and RM-1 cells were seeded in a 96-well plate at a density of
5×103 cells per well. After one day, PBS or different
concentrations of IL-28B (0, 125, 250, 500, and 1000 ng/mL) were added
for treatment. The CellTiter-Lumi™ Luminescent Cell Viability Assay Kit
(Beyotime Biotechnology, Shanghai, China) was used to measure cell
viability at 48 h and 72 h, according to the manufacturer’s
instructions.
2.6 Isolation and culture of bone marrow-derived macrophages