2.2 Cell lines
MC38 (a mouse colon tumor cell line) and RM-1 (a mouse prostate tumor
cell line) cell lines were purchased from ATCC (Manassas, VA, USA).
These cells were maintained in DMEM supplemented with 100 U/mL
penicillin, 100 mg/mL streptomycin, and 10% fetal bovine serum (FBS).
DMEM and FBS were purchased from Gibco (Grand Island, NY, USA).