2.11 Western blot analysis
Proteins were extracted from different treatment groups of M2 macrophages. Protein concentration was determined using an Enhanced BCA Protein Kit (Beyotime Biotechnology, China). Cell extracts containing equal amounts of total protein were subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the resolved proteins were transferred to polyvinylidene fluoride membranes (Invitrogen). After blocking with 3% BSA for 2 h at room temperature, the membranes were incubated with primary antibody overnight at 4 °C. The next day, the membrane was incubated with the corresponding secondary antibody for 2 h. Finally, an enhanced chemiluminescence kit was used to detect immunoreactivity.