2.4 Apoptotic cell analysis
To demonstrate whether IL-28B directly promotes tumor cell apoptosis,
MC38 and RM-1 cells were cultured. When tumor cells are in logarithmic
growth, they were paved to 12-well plates (3×105cells/mL) and treated with phosphate-buffered saline (PBS) or IL-28B
(100, 200, and 400 ng/mL) for 48 h and 72 h. Cells were stained with an
apoptosis kit (KeyGen Biotech,
Nanjing, China) according to the
manufacturer’s protocol and detected using flow cytometry.