2.3 Mouse tumor models and IL-28B treatments
To establish subcutaneous tumor-bearing mouse models, C57BL/6 mice were injected with MC38 cells on the right flank. When tumors were visible, tumor volume was measured with a caliper every two days. Mice were sacrificed 2–3 weeks following tumor cell inoculation. Tumors were collected and weighed. Tumor volume was determined using the following formula: tumor volume (mm3) = length/2 (mm) × width2 (mm2). In the subcutaneous tumor-bearing mouse model, IL-28B (2.5 μg/mouse) was injected into the tissue surrounding the mouse tumor once a day, starting from the 8th day when tumors were visible. The IL-28B was purchased from Sino Biological (Beijing, China).