3.4 IL-28B reduces the expression of Arg-1 and TGF-β in M2 macrophages
We used gene expression and ELISA analyses in BMDMS to confirm IL-28B effects on M2 macrophages. Compared with the control group in the ELISA analysis, 400 ng/mL of IL-28B significantly inhibited the secretion of Arg-1 and TGF-β in M2 macrophages (Fig. 4A). We also detected the expression of M2 macrophage-related genes (Arg-1 and TGF-β) using qPCR. In the IL-28B treatment group, the expressions of M2 macrophage-related genes were significantly downregulated (Fig. 4B). In summary, IL-28B inhibited the expression of M2-related genes and cytokine secretion.