3.3 IL-28B inhibits polarization of BMDMs to M2 macrophagesin vitro
To study the effect of IL-28B on M2 macrophages, we treated BMDMs with IL-28B. The treatment process is illustrated in Fig. 3A. The proportion of M2 macrophages was detected using flow cytometry after 24 h of BMDM polarization. M2 macrophages were defined as CD11b+F4/80+CD206+cells. We found that, compared with the control group, different concentrations of IL-28B reduced the relative proportions of M2 macrophages (Fig. 3B-C). We also explored whether IL-28B did affect M1 macrophages in vitro . Results showed that IL-28B had no effect on M1 macrophages (Fig. 3D-E). These findings suggest that IL-28B directly affects M2 macrophage polarization. The effect of IL-28B on M2 macrophages might play an important role in treating colon cancer.