3.1 IL-28B effectively inhibits tumor growth in vivo
It has been reported that IL-28B exerts anti-tumor effects in liver
cancer14 and lung cancer19. However,
the role of IL-28B and the mechanism of colon cancer remain unclear. To
detect the effect of IL-28B on MC38 murine colon tumors, we built a
mouse MC38 subcutaneous tumor model. After a week, IL-28B was injected
into the surrounding tumors every day. We formulated this schedule (Fig.
1A). In MC38 tumor-bearing mice, the IL-28B treatment group (IL-28B)
significantly inhibited tumor growth. The tumor size was smaller in the
IL-28B group than in the PBS control group (Fig. 1B). The volume and
weight of tumors were significantly reduced compared with those in the
PBS control group (Fig. 1C, D). In the H&E staining analysis, tumor
necrosis in the IL-28B group remarkably increased (Fig. 1E). These data
suggest that IL-28B effectively inhibited MC38 colon tumor growthin vivo .