3.5 IL-28B downregulates STAT3 and JNK signaling pathways in BMDMs
We evaluated the effect of IL-28B on STAT3 and mitogen-activated protein kinase (MAPK) pathway activation in M2 macrophages. As shown in Fig. 5, compared with the control group, IL-28B treatments (200 and 400 ng/mL) significantly inhibited STAT3 and JNK phosphorylation. We demonstrated that IL-28B treatments significantly and dose-dependently inhibited STAT3 and JNK phosphorylation compared with the control group.